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Poly(A) RNA Selection Kit V1.5 - Highly specific enrichment for polyadenylated transcripts

Poly(A) RNA Selection Kit V1.5

The Poly(A) RNA Selection Module enables rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples. Optimized oligo(dT) Magnetic Beads (MB) allow isolation of polyadenylated transcripts within ~one hour.

The Poly(A) RNA Selection Kit V1.5 is available as a convenient bundle with Lexogen’s CORALL RNA-Seq Library Prep Kit for streamlined mRNA sequencing or as standalone kit for 96 reactions (Cat. No. 157.96).

Performance

Lexogen’s Poly(A) RNA Selection Module effectively selects polyadenylated transcripts from total RNA for seamless mRNA sequencing.
Highly Specific for Poly(A) RNA
Other RNA species (rRNA and tRNA) do not contain poly(A) sequences and therefore will not bind to the oligo(dT) beads.
Figure 1. Bioanalyzer traces of Universal Human Reference Total RNA (UHRR, red trace) and poly(A) selected RNA from 5 µg UHRR (blue trace).

The isolated mRNA can directly be used for RNA-Seq library preparation (e.g., CORALL RNA-Seq Library Prep Kits), SAGE, CAGE, cloning, microarrays, cDNA synthesis, and others.

Workflow

Step1:

The total RNA Is briefly denatured and magnetic beads are aliquoted and washed.

The polyadenylated 3’ ends present in most mRNAs
are hybridized to oligodT beads.

Any RNA without poly(A) stretches, such as rRNA
and tRNA,will not be captured by the oligodT beads
and will be washed away.

The poly(A) RNA is eluted from the olidodT beads
by heating in water.

FAQ

Frequently Asked Questions

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Please also check our General Guidelines and FAQ resources!

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Downloads

Safety Data Sheet

If you need more information about our products, please contact us through support@lexogen.com or directly under +43 1 345 1212-41.

Ordering Information

Cat. No. Product Name
157.96Poly(A) RNA Selection Kit V1.5

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