High-throughput Drug Sequencing
Extraction-free gene expression screening directly from lysates
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High-throughput Drug Sequencing
Traditional high-throughput screening often relies on visual markers, which can be challenging to quantify and limit the scope of analysis. RNA-Seq based screening offers a comprehensive view of the transcriptome at scale; providing quantitative data for the discovery of genes and pathways affected by active compounds independent of visual detection. Lexogen’s solutions for high-throughput drug screening are lysate compatible, ultra-scalable, and applicable for primary cells – down to single cells.
Benefits
- Highly affordable
- Lysate compatible
- Ultra-scalable
- Ideal for ultra-low input, from <1 to 100s-1000s of cells
- Designed for drug discovery & development stages
Applications
- Compound screenings during lead discovery
- Single-cell CRISPR perturbation screens for target identification
- Dose-dependent pertubation studies using small molecules or RNA therapeutics, e.g., siRNAs
- Cell-based unbiased high-throughput screening
- Identification of disease-associated pathways as potential drug targets
- Mode-of-action indication
- Biomarker identification
Deliverables
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Rseqc (.txt)
- Gene counts and counting summary (.tsv)
- Differential expression and DE summary (.tsv)
- Multiqc reports – raw, trimmed, alignment, rseqc (.html)
- PCA plot
- Deliverables summarized in a PDF report
Working with us
We offer different NGS Services packages. You can send us lysates (lysis buffer is provided) or purified RNA (Start from Library Prep). It is also possible to do “Sequencing only” projects of your prepared and pooled libraries, all library types are accepted. Each service package can be further customized based on your sample type and requirements. Data Analysis is offered for each of our NGS services packages or as a service on its own (Lexogen Bioinformatics Services). We are also specialized in Custom Bioinformatics Solutions, e.g., for new pipeline developments.
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Extraction
- Not required.
- Plate-format preferred.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC included.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
