A new QuantSeq 3’mRNA-Seq Library Prep is the best alternative for microarrays and conventional RNA-Seq for gene expression analysis and eQTL studies, and a great tool for detection of the 3’UTR.
Lexogen has launched the QuantSeq 3’mRNA-Seq Library Prep kit. This protocol generates Illumina-compatible libraries of the sequences close to the 3’ end of the polyadenylated RNA. Only one fragment per transcript is produced, therefore no length normalization is required. This results in extremely accurate gene expression values, making QuantSeq the best alternative for microarrays and conventional RNA-Seq for gene expression and eQTL studies. External barcodes allowing up to 96 samples to be sequenced per lane on an Illumina flow cell are included in the kit. This high level of multiplexing allows significant saving of costs as the length restriction in QuantSeq saves sequencing space. Moreover, QuantSeq has very simple workflow and takes 4.5 hours including 2 hours of hands-on time. The starting material can be as less as 5 ng of total RNA. The protocol maintains strand-specificity of more than 99.9% thus allowing efficient detection of the antisense transcripts, overlapping and fusion genes. Learn more at the QuantSeq product page.