Description

The SENSE mRNA-Seq Kit is an all-in one strand-specific mRNA-Seq library preparation protocol. SENSE uses total RNA input and is available for Illumina (V2 reagents) and Ion Torrent platforms. Barcode Sets are included for Illumina (i7 indices, 7001-7096), and sold separately for Ion Torrent. For Illumina there is the additional possibility to use four external i5 indices (Dual Indexing Add-on Kit, Cat. No. 047) that allows unique indexing and multiplexing of up to 384 samples.

NOTE: As of February 2019 barcode plates have been renamed to Lexogen i7 6 nt Index Set (7001-7096). An evaluation tool to check the color balance of index subsets is available here.

Features and Application:

  • gene expression profiling
  • whole transcriptome sequencing
  • alternative splicing
  • discovery and quantification of antisense transcripts and overlapping genes

System Compatibility:

  • HiSeq 2000/2500/3000/4000
  • NextSeq 500/550
  • MiSeq
  • Genome Analyzer
  • Ion Proton System
  • Ion PGM System

Kit Content for Illumina:

  • Reagent Box
  • Poly(A) RNA Selection Kit
  • Lexogen i7 6 nt Index Set (formerly named: i7 Index Plate) with i7 indices 7001 – 7008, 7001-7024 or 7001-7096 depending on the kit size (8, 24, 96 preps)
  • Purification Module with Magnetic Beads

Kit Content for Ion Torrent:

  • Reagent Box
  • Poly(A) RNA Selection Kit
  • Buffers and Purification Columns