Description

The QuantSeq REV Kit is an all-in-one library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.

With this kit the exact 3’ end can be pinpointed as the NGS reads directly start from the poly(A) tail using a Custom Sequencing Primer (CSP) which is included in the kit. This version of the kit is also recommended for paired-end sequencing.

For multiplexing of up to 384 samples/lane, the Lexogen i5 6 nt Dual Indexing Add-on Kit (Cat. No. 047) can be used that allows the dual indexing with four additional i5 indices.

NOTE! QuantSeq Kits are provided with free access to the BlueBee Data Analysis Pipeline. Before starting your library prep, please check the online Frequently Asked Questions (FAQ 1.28), or contact support@lexogen.com to see if your species is available on the BlueBee Platform. Lexogen can add reference genomes for new species to the BlueBee Data Analysis Pipeline upon request. Please note this will incur a fee.
New reference genome implementation timelines are dynamic due to feasibility checks and formatting adjustments and therefore cannot be precisely guaranteed. We recommend submitting your request as early as possible prior to sequencing your libraries. New genome upload acceptance is also subject to change in the case of compatibility issues. For all related enquiries, please contact support@lexogen.com.

The old version of QuantSeq REV (Cat. No. 016.24, 016.96 and 016.2×96) is available on demand. If you wish to order it, please contact info@lexogen.com.

The QuantSeq data analysis pipeline is available on the OnRamp Platform ROSALIND, which allows Lexogen’s customers to analyze, interpret and collaborate globally on differential gene expression analysis without the need for specialized bioinformatics or programming skills. Till the end of 2020 every Lexogen’s customer purchasing a QuantSeq kit (FWD or REV) is eligible for 24 free runs on ROSALIND. Contact support@lexogen.com to activate your free runs. For additional runs, please contact your local sales representative and support@lexogen.com.

Features and Application:

  • Gene expression studies
  • 3’ UTR tagging
  • Alternative polyadenylation
  • eQTL analysis
  • Suitable for low quality RNA (including FFPE samples)
  • From as low as 10 ng total RNA

Kit Contents:

  • Reagent Box
  • Lexogen i7 6 nt Index Set (formerly named: i7 Index Plate) with i7 indices 7001-7024; 1x or 2x 7001-7096 depending on the kit size (24, 96 or 192)
  • Purification Module with Magnetic Beads

System Compatibility:

Optional PCR Add-on Kit (96 rxn)

The PCR Add-on Kit is highly recommended since PCR cycle numbers depend on the input material (type, quality, and amount of RNA) and may differ from the values given in the User Guide. Using the PCR Add-on Kit over- or undercycling of libraries can be prevented. Please note that the user will need to provide SYBR Green I (or an equivalent fluorophore) for the qPCR assay.