We are very happy to share with you the videos of our technical talks from AGBT 2016 which are now available at youtube. Do not hesitate sharing them with those who might be interested.

Feel free to contact us at info@lexogen.com for any questions about these talks and not only.

Torsten Reda, PhD

Lexogen, Vienna, Austria

Spike-in RNA Variants (SIRVs): External transcript isoform controls in RNA-Sequencing

Meaningful spike-in controls which mimic the complexity of transcriptomes become increasingly important for RNA-Seq experiments when it comes to compare not only samples within individual experiments but data from different experiments, be it from different sites, or simply the very same experimenter but over the course of fast technological developments in NGS. We present, with the artificial Spike-in RNA Variants (SIRVs), a new core module which was derived from human model genes, and covers start- and end-site variations, alternative splicing, overlapping genes, and antisense transcription in a redundant condensed format. We comment on design principles, challenges in producing in vitro transcripts and mixtures, and show examples of how the SIRV quantifications allow to determine the comparability of sequencing experiments.

Dalia Daujotyte, PhD

Global Scientific Liaison Manager
Lexogen, Vienna, Austria

RNA-Seq as an efficient tool for gene expression profiling

With the rapid development of NGS technologies, RNA-Seq has become the new standard for transcriptome analysis. Although the price per base has been substantially reduced, sample preparation, sequencing, and data processing still remain major cost factors in high-throughput screenings. The QuantSeq technology addresses these issues by providing an easy protocol to generate highly strand-specific NGS libraries close to the 3’ end of polyadenylated RNAs within 4.5 hours, requiring only 0.1 – 500 ng of total RNA input and less bioinformatics efforts than standard RNA-Seq. It is the method of choice for fast, affordable and accurate gene expression detection, quantification and 3’ UTR studies. The protocol can be readily modified for targeted sequencing and include molecular barcoding. In our workshop we will present QuantSeq technology and specify its advantages over the standard RNA-Seq for gene expression profiling and 3’ UTR studies from users’ case studies.