More than 1100 RNA scientists gathered in Berkeley, California, this week, and Lexogen is delighted to have sponsored this Annual Meeting of the RNA Society. 6 days packed with cutting-edge science on our most favorite molecule shed light on functions and mechanisms in splicing, translation, RNA turnover, 3’-end formation etc.
In the Lexogen-sponsored morning seminar “When to switch to 3’ mRNA-Seq?”, diverse applications of the QuantSeq 3’ mRNA-Seq library preparation were introduced, from gene expression quantification – also in whole blood samples – to poly(A) site detection and the use as a highly efficient library preparation for the metabolic RNA sequencing technology SLAMseq. The availability of 96 x 96 dual index barcodes, Unique Molecular Identifiers (UMIs) and full automation complement this tag-profiling offering. Brid Ryan of the National Cancer Institute presented her findings on alternative adenylation in lung cancer based on data produced with the QuantSeq kit. If you want to know more about alternative adenylation as a form of proto-oncogenic activation and the impact of smoking on poly(A) site selection in epithelial cells, watch out for the upcoming publication of her group.
Lexogen’s Spike-In RNA Variants (SIRVs) were featuring frequently in talks based on Oxford Nanopore sequencing results. These external isoform controls are ideal for measuring qualitative and quantitative performance parameters of long-read sequencing, in both, direct RNA-Seq and cDNA sequencing (see also Garalde et al, 2018 and this ONT blog entry). A Nanopore-specific application is the measurement of poly(A) tail length, which is correlated with ionic current dwell time; also here, the fixed 30-nt poly(A) length of the SIRV isoforms provided for an external control.
Elena Galimberti presented the Leeb and Ameres labs’ findings on the “Role of nonsense-mediated mRNA decay in the exit of pluripotency” using the SLAMseq metabolic RNA-Seq technology that Lexogen provides in kit format and as a service.
Lexogen was also happily sponsoring the Junior Scientists’ bike tour along the San Francisco shoreline, from downtown to Pier 39 with its seals population and all the way to the Golden Gate Bridge. This follows Lexogen’s approach to sponsor more than 20 “RNA Salons” and RNA Clubs world-wide that give graduate students and PostDocs the opportunity to present their findings, forge collaborations and to ensure good science by communication and transparency.
We look forward to staying in touch with the large number of visitors at our booth and the RNA “tribe” in general, at other conferences, at local Lexogen seminars, and in personal communication. See you in 2019 in Krakow, Poland!