Lexogen and Bluebee co-hosted a webinar last week to introduce the latest updates in the Lexogen-Bluebee sample-to-data mRNA analysis platform, including the new QuantSeq UMI data analysis pipeline. As a part of the webinar the invited speaker Dr. Subham Dasgupta from the David Volz Lab at the University of California, Riverside has presented application of Lexogen’s QuantSeq 3′ mRNA-Seq Library Prep Kit and the integrated data analysis pipeline from Bluebee to unravel the impact of toxicity-induced changes in embryo development.
Reference: Dasgupta S, Cheng V, Vliet SMF, Mitchell CA, Volz DC. 2018. TDCIPP exposure during early-blastula alters the normal trajectory of zebrafish development. Environmental Science and Technology. 52 (18), pp 10820–10828.
Dr. Dasgupta’s Biography:
Dr. Dasgupta is a Postdoctoral Scholar specializing in environmental toxicology in Dr. David Volz’s laboratory at University of California, Riverside. He completed his BS in Human Physiology (2008) and MS in Environmental Sciences (2010) from University of Calcutta in India. In 2011, he joined the PhD program in Marine and Atmospheric Sciences at the Stony Brook University in New York, working with Dr. Anne McElroy on investigating mechanisms of toxicity of crude oil and dispersants used during the Deepwater Horizon oil spill on developing fish embryos and larvae. After graduating in 2016, Dr. Dasgupta joined Dr. Volz’s laboratory, where he is currently using next-generation sequencing, reverse genetics and other molecular and immunological tools to study the mechanisms of toxicity of flame retardants on early development of zebrafish embryos.