Lexogen’s established RNA extraction protocol is now also available for blood samples.
SPLIT RNA Extraction Kit has already been used in numerous studies where it was applied to purify RNA from different organisms including animal (e.g. mouse, human) and plant tissues (e.g. A. thaliana, Picea abies), insects (e.g. Drosophila), cell lines (e.g. human), fluid samples (e.g. plasma), and others (jellyfish, fungi, bacteria). We are happy to announce that with the launch of SPLIT for Blood the range of SPLIT applications has been extended further. This highly efficient and easy method can now be used also for purification of RNA directly from blood samples.
The SPLIT RNA Extraction workflow enables easy, fast, and reliable extraction of RNA that is free of genomic DNA contamination (without DNase treatment which may damage RNA). The RNA can be recovered as total RNA or split into a large and a small RNA fraction, facilitating the analysis of e.g., mRNA and miRNA from the same sample. The obtained RNA is ideal for demanding applications such as Next Generation Sequencing (NGS) library preparation, full-length cDNA generation, RT-PCR, or microarray analysis.
The SPLIT RNA Extraction Kit for Blood was specifically developed for RNA extraction from fresh human blood and enables concomitant depletion of globin mRNAs from blood samples in low volumes (50 – 250 µl).
RNA Sequencing is a demanding application with its own special input RNA requirements. Samples extracted with the SPLIT kits deliver the whole range of RNA sizes for RNA-Seq, from miRNAs (down to 17 nucleotides) to mRNAs of over 10,000 nucleotides length.
The efficient depletion of the predominant globin mRNA species from the blood RNA samples frees up sequencing space and results in increased gene discovery rates (see Figure below), thereby saving sequencing costs.
Figure. Increased gene detection in human blood QuantSeq libraries using SPLIT RNA Extraction Kit for Blood. RNA was extracted from fresh human blood using the SPLIT (blue) and SPLIT for Blood (green) protocols. RNA-Seq libraries were prepared with Lexogen’s QuantSeq 3’ mRNA-Seq (FWD) kit, and the read fraction mapping to globin mRNAs was calculated. A) Gene Discovery Plot. The number of discovered genes was calculated from CPM (Counts Per Million) normalized read counts (threshold >0.5 CPM). B) Venn Diagram depicting the number of genes detected in libraries from RNA extracted using the SPLIT and SPLIT for Blood protocols and the overlap between the two.