SQUARE

SQUARE stands for Selective Quantitative Amplification of RNA and enables hypothesis free identification and quantification of all known and unknown full length transcripts. SQUARE is based on the selective amplification of full length RNAs based on their unique 5’ start and 3’ terminating sequences. SQUARE in this manner subdivides splice variants of families of transcripts in different so-called matrix fields. Individual matrix fields can then be sequenced with Next Generation Sequencing technologies.