Complete Transcriptome Profiling

Transcriptome analyses aim to detect biologically relevant sequence and expression differences. Recent advances in Next Generation Sequencing (NGS) technologies constitute a breakthrough in the scope with which such differences can be detected.

A critical third dimension in transcriptome analyses is variation in transcript splicing and processing. For instance, human genes are on average estimated to be expressed in more than eight splice variants. Transcript splicing and processing determine the identity of translated proteins and thus a gene’s biological function.

Lexogen's SQUARE technology subdivides the complete transcriptome and families of splice variants. SQUARE thus empowers Next Generation Sequencing to open up this third dimension and greatly increases the power of transcriptome analysis as it enables the expression analysis and identification of individual splice variants. For an example of how SQUARE empowers transcriptome analyses we refer you to the SQUARE Application Note.