Lexogen, a transcriptomics and Next Generation Sequencing company, is focusing on the development of technologies for complete transcriptome sequencing.

The QuantSeqTM 3’mRNA-Seq Library Prep Kit is a fast, very strand-specific, and cost-effective protocol for sequencing of fragments close to the 3’ end of the polyadenylated RNA. It allows exact tagging of the 3’UTR and produces extremely accurate expression values. Therefore, QuantSeq is the best alternative to microarrays and conventional RNA-Seq in gene expression and eQTL studies.

The SENSETM mRNA-Seq Library Prep Kit generates ready-to-sequence libraries with exceptional strand-specificity from low amounts of total RNA within just 4 hours. The kits are available for Illumina, Ion Torrent, and SOLiD sequencing instruments. Automated SENSE mRNA-Seq Library Prep can be performed on the PerkinElmer Sciclone/Zephyr NGS workstations.

Lexogen's proprietary SQUARETM Technology presents a unique, hypothesis-free approach to reduce the complexity of the transcriptome and empowers Next Generation Sequencing technologies to sequence and quantify all splice variants in which genes are expressed. Lexogen's SQUARE technology will provide unprecedented insight in molecular pathways.

The SPLITTM RNA Extraction Kit enables a fast and highly efficient extraction of RNA that is free of genomic DNA contamination. The RNA can be recovered as total RNA or split into a large and a small RNA fraction, facilitating the analysis of e.g. mRNA and miRNA from the same sample. Thus the protocol is ideal for seamlessly preparing libraries for Next Generation Sequencing of total RNA or its large and small fractions.