Lexogen, a transcriptomics and Next Generation Sequencing (NGS) company, is focusing on the development of technologies for complete transcriptome sequencing.

The QuantSeqTM 3’mRNA-Seq Library Prep Kit is a fast, very strand-specific, and cost-effective protocol for sequencing of fragments close to the 3’ end of the polyadenylated RNA. It allows exact tagging of the 3’UTR and produces extremely accurate expression values. Therefore, QuantSeq is the best alternative to microarrays and conventional RNA-Seq in gene expression and eQTL studies. The QuantSeq 3’mRNA-Seq Library Prep Kits are availabe for Illumina and Ion Torrent sequencing platforms. An automated QuantSeq 3'mRNA-Seq Library Prep for Illumina can be performed on the PerkinElmer Sciclone/Zephyr NGS workstations.

SENSETM Total RNA/mRNA-Seq Library Prep Kits generate ready-to-sequence libraries with exceptional strand-specificity from low amounts of input RNA within a few hours. SENSE mRNA-Seq Library Prep Kits is available for Illumina, Ion Torrent, and SOLiD sequencing platforms. An automated SENSE mRNA-Seq Library Prep can be performed on the PerkinElmer Sciclone/Zephyr NGS workstations. SENSE Total RNA-Seq Library Prep Kit is available for Illumina sequencing platform.

TeloPrimeTM Full-Length cDNA Amplification Kit for generating full-length cDNA from total RNA. It is highly selective for full-length RNA molecules that are both capped and polyadenylated and therefore no cDNA from degraded RNA is being amplified. The full-length cDNA products can be further used for various downstream applications such as NGS, RACE, cloning, microarray probes, and normalization.

Lexogen's proprietary SQUARETM Technology presents a unique, hypothesis-free approach to reduce the complexity of the transcriptome and empowers NGS technologies to sequence and quantify all splice variants in which genes are expressed. Lexogen's SQUARE technology will provide unprecedented insight in molecular pathways.

The SPLITTM RNA Extraction Kit enables a fast and highly efficient extraction of RNA that is free of genomic DNA contamination. The RNA can be recovered as total RNA or split into a large and a small RNA fraction, facilitating the analysis of e.g. mRNA and miRNA from the same sample. Thus the protocol is ideal for seamlessly preparing libraries for NGS of total RNA or its large and small fractions.